| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Minnesota Rush Minnesota Ru - B16 |
0101-07UB16-0032 | Julio Aragon Fantini |
| A2 | Mankato United Soccer Club Mankato Unit - B16 |
0101-05UB16-0020 | Cooper Jones |
| A3 | Kasson-Mantorville Youth Soccer Association KMYSA B16U |
0101-04UB16-0003 | Charles Gierok |
| A4 | New Ulm United Soccer Club NUUSC - B16 |
0101-09UB16-0022 | Wayne Owens |
| A5 | Mississippi Valley Storm Soccer Club Mississippi Valley Storm - B16 |
0101-20UB16-0008 | Josh Iliff |
| A6 | Minnesota Rush Minnesota Ru - B15 01 |
0101-07UB15-0030 | Alvaro Capra |
| A7 | Minnesota Rush Minnesota Ru - B15 02 |
0101-07UB15-0031 | Igor Pacheco Oliveira Pinto |
| A8 | Mountain Lake Mountain Lake - B16 |
0101-23UB16-0005 | Rodrigo Hernandez Deleon |